Biochemistry (Enzyme Kinetics)

[wr1015]

The following kinetic data were obtained for an enzyme in the absence of inhibitor (trial A), and in the presence of two different inhibitors (B and C) at concentrations of 5 mM

http://i78.photobucket.com/albums/j88/wr1015/bchw.jpg

a) Determine Vmax and Km for the enzyme (Assume [Et] is the same for each trial)

the first thing I thought to do was use the relationship that Km = [S] when Vo= 1/2Vmax, however, after posing this question to my professor that is incorrect. I really don't know where to start so any help to get me started on the right track would be immensely appreciated.

[GCT]

Hi, you need to read through the relevant chapter in your text a bit more carefully, it should describe a specific method of plotting to obtain the Vmax and Km hint: This method is probably one of the most important concepts explained within the chapter of which it is mentioned, the Vmax can be obtained from the y-intercept value of the unique plot and the x-intercept should reveal the Km.

[wr1015]

ah yes Lineweaver-Burk plot, thanks