again chromatography!

[mountain]

hi guys!
as you know i am doing the chromatography stuff and am now having the problem with understanding the protocol. it says;

"set a clen after gradient value to 100% buffer B over 2 CV”? :bugeye:
does any of you know what it tries to tell me?

thanks!

[Moonbear]

Does your manual spell out what they mean by "CV" anywhere? I could guess a few things, but probably would guess wrong. What about "clen"...is that a typo or what is actually written (or maybe what's actually written is a typo...been known to happen).

All these column chromatography questions might get better responses in the chemistry forum, since organic chemists use them more frequently (and such methods are almost always taught in organic chemistry classes).