Chromatography Problem: Setting a "Clen After Gradient

  • Thread starter Thread starter mountain
  • Start date Start date
  • Tags Tags
    Chromatography
Click For Summary
SUMMARY

The discussion centers on a chromatography protocol that instructs users to set a "clen after gradient" value to 100% buffer B over 2 CV (column volumes). Participants express confusion regarding the term "clen," questioning whether it is a typographical error or a specific term used in chromatography. Additionally, there is a suggestion to seek further clarification in a chemistry forum, as organic chemists are more familiar with such methodologies.

PREREQUISITES
  • Understanding of chromatography techniques and protocols
  • Familiarity with buffer solutions in chromatography
  • Knowledge of column volumes (CV) in chromatography
  • Basic terminology used in organic chemistry
NEXT STEPS
  • Research the role of buffer B in gradient elution chromatography
  • Learn about column volume calculations and their significance in chromatography
  • Investigate common terminology and potential typos in chromatography protocols
  • Explore organic chemistry forums for expert insights on chromatography methods
USEFUL FOR

Chemistry students, laboratory technicians, and researchers involved in chromatography who seek clarification on protocol terminology and gradient settings.

mountain
Messages
52
Reaction score
0
hi guys!
as you know i am doing the chromatography stuff and am now having the problem with understanding the protocol. it says;

"set a clen after gradient value to 100% buffer B over 2 CV”? :bugeye:
does any of you know what it tries to tell me?

thanks!
 
Biology news on Phys.org
Does your manual spell out what they mean by "CV" anywhere? I could guess a few things, but probably would guess wrong. What about "clen"...is that a typo or what is actually written (or maybe what's actually written is a typo...been known to happen).

All these column chromatography questions might get better responses in the chemistry forum, since organic chemists use them more frequently (and such methods are almost always taught in organic chemistry classes).
 

Similar threads

Replies
2
Views
6K
Replies
2
Views
3K
  • · Replies 8 ·
Replies
8
Views
3K
  • · Replies 1 ·
Replies
1
Views
3K
Replies
11
Views
2K
  • · Replies 6 ·
Replies
6
Views
3K
  • · Replies 16 ·
Replies
16
Views
3K
Replies
13
Views
2K
Replies
6
Views
2K
  • · Replies 2 ·
Replies
2
Views
5K