ryguy
Oct20-04, 09:50 PM
Hey, I'm a newbie here. :smile: Just wanted to say this is a really great site. Lots of informative stuff!
Anyway, my midterm is coming up and I'm having trouble grasping enzyme kinetics. I'm familiar with the Michaelis - Menten equation, but I don't know how this equation is applied since we never went through any practice problems in class. For example here is a question.
An enzyme catalyzes a reaction at a velocity of 20 umoles/min when the concentration of substrate is 0.01M. The Km for this substrate is 1X10^-5M. Assuming that Michaelis-Menten kinetics are followed, what will the reaction velocity be when the concentration of substrate is:
a)1X10^-5M
b)1X10^6M
So for part a:
Km = 1X10^-5M
[S] = 1X10^-5M
Vmax = 20umoles/min????
and we have to find V-inital?
or is V-initial = 20umoles/min?
Help is greatly appreciated!
Anyway, my midterm is coming up and I'm having trouble grasping enzyme kinetics. I'm familiar with the Michaelis - Menten equation, but I don't know how this equation is applied since we never went through any practice problems in class. For example here is a question.
An enzyme catalyzes a reaction at a velocity of 20 umoles/min when the concentration of substrate is 0.01M. The Km for this substrate is 1X10^-5M. Assuming that Michaelis-Menten kinetics are followed, what will the reaction velocity be when the concentration of substrate is:
a)1X10^-5M
b)1X10^6M
So for part a:
Km = 1X10^-5M
[S] = 1X10^-5M
Vmax = 20umoles/min????
and we have to find V-inital?
or is V-initial = 20umoles/min?
Help is greatly appreciated!