Help with understanding about Sordaria

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Discussion Overview

The discussion revolves around the use of the haploid fungus Sordaria in genetics experiments, particularly in relation to meiosis, crossing over, and the applicability of statistical methods used in diploid organisms like Mendel's peas and corn.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested

Main Points Raised

  • One participant questions how a haploid organism like Sordaria can be used to test genetics problems, particularly regarding crossing over and meiosis.
  • Another participant asserts that ascomycetes, including Sordaria, can undergo meiosis and that environmental changes can promote either asexual or sexual reproduction.
  • A participant references a related topic on Aspergillus to suggest that Sordaria also has a dikaryon phase that allows for genetic recombination, thus enabling the study of genetic problems similar to those with diploid organisms.
  • There are repeated inquiries about the procedures that would allow the use of Sordaria for testing genetic problems akin to those studied with Mendel's peas.
  • One participant suggests that to use Sordaria for Mendelian experiments, one must induce the fungus to produce diploid perithecia by combining different haploid strains in the same growth media.

Areas of Agreement / Disagreement

Participants express uncertainty regarding the mechanisms by which Sordaria can be used in genetic testing, with multiple viewpoints on the relevance of its haploid nature and the applicability of statistical methods from diploid species. The discussion remains unresolved with no consensus reached.

Contextual Notes

Limitations include the dependence on environmental factors for inducing sexual reproduction in Sordaria and the need for further clarification on the specific procedures for conducting genetic experiments with this organism.

Ester
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Please help me.
I know that Sordaria is a member of the ascomycete fungi, a group often used in genetics experiments. Yet, Sordaria is haploid.
How can a haploid organism be used to test genetics problems when such things as crossing over require meiosis as the cell goes from a diploid to a haploid stage?
How, then, can we use this haploid organism to test the same genetic problems as with Mendel's peas?
Is it possible to use the same statistical procedures used in diploid species such as corn?
 
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Short answer: Ascomycetes can undergo meiosis. By changing the environment you can encourage either asexual or sexual reproduction.
 
Ester said:
How can a haploid organism be used to test genetics problems when such things as crossing over require meiosis as the cell goes from a diploid to a haploid stage?
You can find insight on your question, in a recent topic about another Ascomycete - Aspergillus (ref recent topic). Both Sordaria and Aspergillus undergo a dikaryon (diploid) phase which leads to genetic recombination.

How, then, can we use this haploid organism to test the same genetic problems as with Mendel's peas?
Is it possible to use the same statistical procedures used in diploid species such as corn?
yes to both, as you will find out by following the above reference, there is a diploid phase in the ascomycete life cycle.
 
How can we use this haploid organism to test the same genetic problems as with Mendel's peas? What types of procedures one would take?
 
Ester said:
How can we use this haploid organism to test the same genetic problems as with Mendel's peas? What types of procedures one would take?
You would need to convince the Sordaria to generate (diploid) perithecia. This is accomplished by bringing to different haploid strains together in the same growing media.This reference may give you some ideas how you might use Sordaria for Mendelian genetic experiments.
 

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