Osmosis is the passage of water from a region of high water

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Discussion Overview

The discussion revolves around the concepts of osmosis and enzyme functionality, particularly focusing on the mechanisms of water movement through semi-permeable membranes and the effects of denaturation on enzyme activity. It includes theoretical explanations and questions regarding the behavior of water potential and enzyme specificity.

Discussion Character

  • Exploratory
  • Technical explanation
  • Conceptual clarification
  • Debate/contested

Main Points Raised

  • One participant defines osmosis as the movement of water from high to low water concentration through a semi-permeable membrane and questions the underlying reasons for this process.
  • Another participant explains that osmosis results from the net movement of water molecules, emphasizing that equal concentrations lead to no net gain of water movement across the membrane.
  • A participant asserts that enzymes are specific and cannot catalyze reactions with other substrates after denaturation, although they speculate about the possibility of renaturation under certain conditions.
  • Another participant challenges the notion of enzyme specificity by mentioning competitive inhibitors that can occupy the active site, suggesting that enzymes may not be entirely specific.

Areas of Agreement / Disagreement

Participants express differing views on enzyme specificity and the effects of denaturation, indicating that multiple competing perspectives exist regarding these topics. The discussion on osmosis also includes varying explanations, suggesting unresolved questions about the mechanisms involved.

Contextual Notes

Some claims about enzyme renaturation depend on specific conditions that are not fully explored in the discussion. Additionally, the definitions and implications of water potential and enzyme specificity are not universally agreed upon, leaving room for further clarification.

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Osmosis is the passage of water from a region of high water concentration through a semi-permeable membrane to a region of low water concentration.
.My question is why high water potential flows water to low water potential. What makes this occurs ?

Also, does enzyme still works after being denatured. For an example, amylase is turn starch to maltose. After amylase is denatured, can it still react with other substances( i know it cannot react with starch)?
 
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Osmosis is a due to net movement. The water molecules move randomly in all directions, and so if you put a semi permeable membrane in the way, the water will move back and forth through it. If the concentrations are equal, as much water will be moving from let's say left to right through the membrane as will be moving right to left, so there is no net gain. If the water on one side of the membrane is impure, it will have less water molecules per unit of volume, an so less that can travel through the membrane. This results in more water entereing from the side with higher water potential than is leaving.
 
No, emzymes are specific so cannot catalyse the hydrolysis of other saccharides. Also denaturation of enzymes means that the precise tertiary and quaternary shape has changed so the substrate cannot fit into the active site. However if enzymes are denatured by an acid / base (not temperature), I think (not sure though) there is a possibility of enzyme renaturation.
 
Enzymes are not 100% specific- this is how competative inhibitors work- they can occupy the same active site as the intended substrate and so reduce the rate of reaction.
 

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