Calculating the Percent of Cytosine in a DNA Molecule with 28% Thymine

In summary, when given the information that a DNA molecule contains 28% thymine, we can calculate that it also contains 28% cytosine, 22% adenosine, and 22% thymine. This is based on the assumption of perfect Watson-Crick base pairing and that the molecule is only composed of the four base pairs: adenine, thymine, cytosine, and guanine.
  • #1
BBboy
11
0
If a DNA molecule containes 28% thymine, what percent of it will be cytosine?

will it be 72%?

please help, thank you
 
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  • #2
You'll have to show some work.

First, is there anymore information that comes with the question? There four different base pair that makes up DNA. Do you think knowing only one will help in solving the percentage of a certain base pair?
 
  • #3
T - thymine bonds with A - adenine
C - cytosine bonds with G - guanine
how can you calculate the percentage of cytosine if it only gives you 28% thymine?
 
  • #4
You know that thymine will only bond with adenine, so the percentages of each one must be related to each other somehow.
The same thing with cytosine and guanine.
 
  • #5
Also assume that DNA is only composed of C,G,T and A. Therefore the total percentages of each individual base pair will equal 100 percent.
 
  • #6
BBboy said:
T - thymine bonds with A - adenine
C - cytosine bonds with G - guanine
how can you calculate the percentage of cytosine if it only gives you 28% thymine?
You can't if that's all the information you're given. Are you sure you weren't given more information than that?
 
  • #7
Actually if you assume perfect Watson-Crick base pairing, then all you need is the percentage of one base, since the base it bonds to will be the same percentage.

So the percentage of A = percentage of T
And 100- percentage of A + percentage of T = percentage of C and T

percentage of C = percentage of T = 1/2 percentage of C and T
 
  • #8
This is a question from P. 232, #17:

http://www.emporia.edu/biosci/genetics/prob9.htm

P. 232, #17. A double stranded DNA molecule is 28% guanosine (G).
a. What is the complete base composition of this molecule?
In double stranded DNA the concentration of guanosine equals the concentration of cytosine, thus the molecule is also 28% cytosine. This leaves 44% for adenosine and thymine combined (1-28%-28% = 44%). As the concentration of adenosine equals the concentrations of thymine, the concentration of adenosine must be 22% and the concentration of thymine must also be 22%.

b. Answer the same question, but assume the molecule is double-stranded RNA.
For double stranded RNA the reasoning is the same except that uracil replaces thymine. Thus for double stranded RNA the base composition is 28% guanosine, 28% cytosine, 22% adenosine, and 22% uracil.
 

What is the formula for calculating the percent of cytosine in a DNA molecule?

The formula for calculating the percent of cytosine in a DNA molecule is (% Cytosine / Total number of nucleotides) x 100.

What is the total number of nucleotides in a DNA molecule with 28% thymine?

The total number of nucleotides in a DNA molecule with 28% thymine can be calculated by first finding the percentage of thymine (28%) and then dividing it by 0.25 (since thymine makes up 25% of the total nucleotides in DNA). This will give you the total number of nucleotides in the DNA molecule.

How can I determine the number of cytosine in a DNA molecule with 28% thymine?

To determine the number of cytosine in a DNA molecule with 28% thymine, you can use the formula (% Cytosine / Total number of nucleotides) x Total number of nucleotides. This will give you the number of cytosine nucleotides in the DNA molecule.

What is the significance of calculating the percent of cytosine in a DNA molecule with 28% thymine?

Calculating the percent of cytosine in a DNA molecule with 28% thymine can provide valuable information about the DNA sequence and can help in understanding the genetic makeup of an organism. It can also help in identifying any potential mutations or variations in the DNA.

What are some possible sources of error when calculating the percent of cytosine in a DNA molecule with 28% thymine?

Some possible sources of error when calculating the percent of cytosine in a DNA molecule with 28% thymine include human error in counting or measuring nucleotides, contamination of the DNA sample, or errors in the laboratory procedures. It is important to carefully follow protocols and perform multiple trials to reduce the risk of error.

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