Sulfate Peaks in IR: Glucosamine HCl & Glucosamine Sulfate 2KCl

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In summary, the conversation discussed the use of FTIR with ATR to scan two different compounds, Glucosamine HCl and Glucosamine Sulfate 2KCl. The spectra were found to be identical, causing confusion about where the peaks of a sulfate group would appear in the IR. The conversation also mentioned that the stabilized form of glucosamine sulfate is actually a mixture of potassium sulfate and glucosamine hydrochloride. The two main infrared active modes of vibration for sulfate ion were also described, but they can be easily overlooked due to the strong absorptions of organic molecules and biomolecules in the same regions. To better differentiate the sulfate signals, it may be necessary to narrow the scan window and decrease the slit
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ldc3
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I scanned 2 different compounds (Glucosamine HCl and Glucosamine Sulfate 2KCl) using an FTIR with ATR and their spectra were identical :confused:. Where would the peaks of a sulfate group appear in the IR?
 
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Sulfate ion in inorganic salts and in simple crystal environments is exactly tetrahedral. Because of this high symmetry there are only two infrared active modes of vibration. A very strong band at around 1100 cm-1 is composed mostly of a S--O stretch, and a moderately strong band at around 650 cm-1 is mainly composed of an umbrella type motion of the other three oxygen atoms -- largely a bending vibration. In a more complicated crystal environment these bands may be shifted (up to about 50 cm-1), broadened (out to a FWHH around 100 cm-1) or split.

Unfortunately, these two regions, 1100 cm-1 and 650 cm-1 co-incide with those where a fairly complicated set of rather strong infrared absorptions appear for most organic molecules and biomolecules. Although the two sulfate signals are quite strong in themselves, they would be easily overlooked if they were broadened and hidden under a lot of organic molecule peaks
 
  • #4
It looks like I will need to narrow my scan window and decrease the slit width to see if there are any differences. Thank you.
 

1. What is the difference between glucosamine HCl and glucosamine sulfate 2KCl?

Glucosamine HCl and glucosamine sulfate 2KCl are two different forms of glucosamine, a compound used in dietary supplements for joint health. The main difference between them is the presence of sulfate molecules in glucosamine sulfate 2KCl. This means that glucosamine sulfate 2KCl contains both glucosamine and sulfate, while glucosamine HCl only contains glucosamine. The sulfate molecules are believed to help with the absorption and effectiveness of glucosamine.

2. Why are sulfate peaks important in IR analysis of glucosamine HCl and glucosamine sulfate 2KCl?

Sulfate peaks are important in IR (infrared) analysis of glucosamine HCl and glucosamine sulfate 2KCl because they indicate the presence of sulfate molecules. This is particularly important for differentiating between the two forms of glucosamine, as glucosamine sulfate 2KCl should have significantly higher sulfate peaks compared to glucosamine HCl.

3. What is the significance of sulfate peaks in the identification of glucosamine HCl and glucosamine sulfate 2KCl?

The presence of sulfate peaks in IR analysis is significant in the identification of glucosamine HCl and glucosamine sulfate 2KCl because it confirms the presence of sulfate molecules, which differentiate between the two forms of glucosamine. This information is important for quality control and ensuring the proper labeling of dietary supplements.

4. Are there any other compounds that could produce similar sulfate peaks in IR analysis of glucosamine HCl and glucosamine sulfate 2KCl?

While sulfate peaks are primarily used to differentiate between glucosamine HCl and glucosamine sulfate 2KCl, there are other compounds that could potentially produce similar peaks, such as chondroitin sulfate or other sulfate-containing compounds. However, these compounds would likely have different peak intensity and patterns compared to glucosamine sulfate 2KCl, allowing for differentiation.

5. Can IR analysis of sulfate peaks be used to determine the purity of glucosamine HCl and glucosamine sulfate 2KCl?

IR analysis of sulfate peaks can give some indication of the purity of glucosamine HCl and glucosamine sulfate 2KCl, as higher levels of sulfate peaks would suggest a higher concentration of sulfate molecules. However, other analytical techniques such as HPLC (high-performance liquid chromatography) may be more accurate for determining the purity of these compounds.

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