What is the purpose of using Concanavalin A coated dishes in cell imaging?

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In summary, Concanavalin A coating is a process in which the protein is used to coat a surface for the specific binding of molecules or cells. This process involves preparing a solution of the protein, applying it to the surface, and washing off any excess before use in experiments. It is commonly used in cell adhesion, sorting, and protein purification experiments, as well as in biosensors and the study of glycosylated proteins. However, there are limitations and considerations, such as the protein's sensitivity to high temperatures and the need for a clean surface for accurate results.
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nancy189
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Can anyone tell me why are Concanavalin A coated dishes used in cell imaging?

Nancy
 
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nancy189 said:
Can anyone tell me why are Concanavalin A coated dishes used in cell imaging?

Nancy

A laboratory based biologist could give you a more detailed answer, but Concanavalin A is apparently used to bind glycosul and mannosyl groups.

http://jura.wi.mit.edu/sabatini_public/fly_array/ConA.htm
 

1. What is Concanavalin A coating?

Concanavalin A coating is a process in which the protein concanavalin A is used to coat a surface, typically a glass or plastic surface. This coating is used in various scientific experiments and procedures to facilitate the binding of specific molecules or cells.

2. How is Concanavalin A coating performed?

The process of Concanavalin A coating involves first preparing a solution of the protein, then applying it to the desired surface and allowing it to dry. The surface is then washed to remove any excess protein and is ready for use in experiments.

3. What is the purpose of Concanavalin A coating?

The purpose of Concanavalin A coating is to provide a surface for the specific binding of molecules or cells. This is useful in experiments where certain proteins or cells need to be isolated or studied separately from other components in a sample.

4. What types of experiments use Concanavalin A coating?

Concanavalin A coating is commonly used in experiments involving cell adhesion, cell sorting, and protein purification. It is also used in biosensors and in the study of glycosylated proteins.

5. Are there any limitations or considerations when using Concanavalin A coating?

Yes, there are some limitations and considerations to keep in mind when using Concanavalin A coating. For example, the protein can lose its activity if exposed to high temperatures or pH levels. Additionally, the coated surface must be kept clean and free of contaminants to ensure accurate results in experiments.

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