How Do You Calculate Total Absorption of a Sample from Its Absorbance Spectrum?

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In summary, total absorption of a sample is the amount of energy absorbed by a sample when exposed to a specific type of radiation. It is typically measured using a spectrophotometer and can be affected by factors such as sample thickness and composition, type and energy of radiation, and sample properties. Total absorption is important in scientific research as it provides information about a sample's properties and behavior. It can be manipulated by changing the properties of the sample or radiation, as well as external factors like temperature and pressure.
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Rositta
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Hi, does anyone know how to calculate the total absorptin of a sample from its labdam-absorbance spectrum?
I found a paper where the author find it as the average of the data, another one numerically integrate the spectrum over the whole lambda range.. but the two methods give different results... what's the corecct way?
 
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Average of what data?

An integration over the wavelength spectrum, with the spectral intensity of the light source as weight, should give the correst result.
 

1. What is total absorption of a sample?

Total absorption of a sample refers to the amount of energy absorbed by a sample when exposed to a specific type of radiation, such as light or X-rays. It is a measure of the sample's ability to absorb and convert the incoming energy into a different form, such as heat.

2. How is total absorption of a sample measured?

Total absorption of a sample is typically measured using a spectrophotometer, which measures the amount of light that passes through the sample. The difference in light intensity before and after the sample allows for the calculation of the total absorption. Other methods, such as X-ray absorption spectroscopy, can also be used for measuring total absorption in specific types of samples.

3. What factors affect the total absorption of a sample?

The total absorption of a sample can be influenced by various factors, including the thickness and composition of the sample, the type of radiation used, and the energy of the radiation. The chemical and physical properties of the sample, such as its molecular structure and density, can also play a role in its total absorption.

4. Why is total absorption of a sample important in scientific research?

Total absorption of a sample is important in scientific research because it can provide valuable information about the properties and behavior of a sample. It can be used to identify and quantify the substances present in a sample, as well as to study the interactions between the sample and the radiation. Total absorption measurements can also be used to monitor changes in a sample over time, such as in chemical reactions or biological processes.

5. How can total absorption of a sample be manipulated?

The total absorption of a sample can be manipulated by changing the properties of the sample or the radiation used. For example, altering the chemical composition or thickness of the sample can affect its total absorption. Similarly, changing the energy or wavelength of the radiation can also impact the total absorption. Additionally, external factors such as temperature and pressure can also influence the total absorption of a sample.

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