- #1
sameeralord
- 662
- 3
1. Ok in the plasmid we used the ECOR1 to create sticky ends. Now my question is when we add DNA ligase, why don't these ends stick back again. How are we able to add new DNA piece in between. Basically what I'm saying is when ECOR 1 creates sticky ends in plamids, why do they separate the plamid, don't the sticky ends stick back together.
2. Also why can't DNA ligase bind the blunt ends, can't it fix the phosphodiester bond there.
Thanks