Isoelectric Point: Calculating Val-Lys-Glu-Asp-Phe

[Touchme]

Hi,


How do I find the isoelectric point for an entire protein sequence? Such as Val-Lys-Glu-Asp-Phe.

 

[biotechatrock]

try at http://isoelectric.ovh.org

 

[Blueshift5]

The isoelectric point (pI) of a protein is the pH at which the net charge of the protein is zero. To calculate the pI of a protein sequence, you can use a tool called a pI calculator. This tool takes into account the amino acid composition and their respective pKa values to determine the pI.

For the protein sequence Val-Lys-Glu-Asp-Phe, the pI calculator would take into account the pKa values of the side chains of each amino acid, as well as the N-terminus and C-terminus. The pKa values of these groups are typically around 2 for the N-terminus and 9 for the C-terminus. The pI calculator would then use an algorithm to determine the pH at which the net charge of the protein is zero.

It is important to note that the pI of a protein is an approximation and can vary depending on the environment and interactions with other molecules. Additionally, some proteins may have multiple pI values due to the presence of multiple charged amino acids.

In conclusion, to find the pI for a protein sequence, you can use a pI calculator that takes into account the pKa values of the amino acids and terminal groups. This will give you an approximate pH at which the net charge of the protein is zero.