- #1
vande060
- 186
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Homework Statement
In the protocol for the bradford method I am told to prepare two blanks of water of .15M NaCl. I don't understand why I need two of them. Don't I just need one of them for placing into the photometer after I have set 0% transmission to set 100% transmission by.
should I put some of the coomassie brilliant blue, in one of these, both of them, or none of them?
I really don't get why I need two of them, any help would be appreciated