What's mock methylation?


by hivesaeed4
Tags: epigenetics, methylation, mock
hivesaeed4
hivesaeed4 is offline
#1
Feb5-14, 01:43 AM
P: 217
Can anyone explain mock methylation. The best explanation that I've been able to coax out of the internet is (incubation with methylase in the absence of SAM (S-adenosyl methionine)). I don't know how this sort of methylation gets done, what gets added onto cytosine and how mock methylation differs from actual methylation. Help, please.
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Ygggdrasil
Ygggdrasil is offline
#2
Feb5-14, 10:21 AM
Other Sci
Sci Advisor
P: 1,342
In what context did you encounter the term "mock methylation." There are many different types of methylation reactions in biology (e.g. proteins, for example the tails of histone proteins, can also be methylated). My guess would be that these mock methylation reactions are just negative control reactions.
hivesaeed4
hivesaeed4 is offline
#3
Feb13-14, 09:31 AM
P: 217
I found out that as well i.e. mock methylation are basically negative control reactions. Thanks Ygggdrasil for confirming it.


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