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g07l0989
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how would you prepare a 100ml of a 0.1M sodium phosphate buffer containing 0.5M NaCl,pH7.6 using henderson hasselbach equation??what chemicals would you use n how would you go about it
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To prepare a 0.1M sodium phosphate buffer, you will need to use the formula C1V1 = C2V2, where C1 is the initial concentration (0.1M), V1 is the initial volume (unknown), C2 is the desired concentration (0.1M), and V2 is the desired volume (100ml). Simply rearrange the formula to solve for V1, which will give you the initial volume of sodium phosphate needed for the buffer.
It is important to use a calibrated balance to accurately measure out the correct amount of sodium phosphate. The mass of sodium phosphate needed can be determined by multiplying the molar mass of sodium phosphate (120.08 g/mol) by the number of moles needed (0.1 mol) and converting the result to grams. Make sure to use a clean and dry weighing boat or container to hold the sodium phosphate.
No, it is important to use only high-quality water, such as deionized or distilled water, to prepare the buffer. Tap water or other types of water may contain impurities that can affect the accuracy and effectiveness of the buffer.
The pH of a buffer can be adjusted by adding small amounts of a strong acid or base. For a sodium phosphate buffer, you can use either phosphoric acid or sodium hydroxide to adjust the pH. It is recommended to use a pH meter or indicator paper to monitor the pH and make small adjustments until the desired value is reached.
Yes, you can store the prepared buffer in a sealed container at room temperature for a few days. However, it is recommended to prepare a fresh batch of buffer for each experiment to ensure the accuracy and consistency of your results.