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Zellbiologie
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Hello! could you help me with this? I´m from biology field and have some difficulties with the following calculation: I want to estimate how much mor UV is coming from a mercury short arc lamp of a fluorescence microscop compared with the sun UV, so that I use the right eye protection for adjusting the microscip light. Maybe just normal sunglasses are enough, but I want to be sure.
When adjusting, you look into the reflected light of the lamp that is inside of a cover. The reflected light on a wall at aprox. 3 m.
So I try to compare the radiant intensity of UV-A -B and C of the mercury short arc lamp (HBO) with those of the sun.
I have the following radiant intensity values per 1000 cd:
220-280 nm UVC = 0,317 W/sr
280-315 nm UVB = 1,165 W/sr
315-380 nm UVA = 2,10 W/sr
Lamp light intensity is 230 cd.
Now I want to convert the values of Radiant intensity (W/sr) in irradiance values. For example the lamp irradiance at a distance of 1 cm. I get 483 mW/cm² for UVA. Is this right?? That means I get much more UV than that coming from the sun at see level (I find in google a value of 0,5 mW/cm²) and should choose not a sunglass but a special UV eye protection.
Thank you very much!
Mara.
When adjusting, you look into the reflected light of the lamp that is inside of a cover. The reflected light on a wall at aprox. 3 m.
So I try to compare the radiant intensity of UV-A -B and C of the mercury short arc lamp (HBO) with those of the sun.
I have the following radiant intensity values per 1000 cd:
220-280 nm UVC = 0,317 W/sr
280-315 nm UVB = 1,165 W/sr
315-380 nm UVA = 2,10 W/sr
Lamp light intensity is 230 cd.
Now I want to convert the values of Radiant intensity (W/sr) in irradiance values. For example the lamp irradiance at a distance of 1 cm. I get 483 mW/cm² for UVA. Is this right?? That means I get much more UV than that coming from the sun at see level (I find in google a value of 0,5 mW/cm²) and should choose not a sunglass but a special UV eye protection.
Thank you very much!
Mara.
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