Determination of single biomolecules

In summary, an electromagnetic field can be used to identify molecules based on their light signatures.
  • #1
divansaray
2
0
how to determine (theoretical, based on physics principles of detection of different fields from molecules without outside influence) individual electromagnetic field from single molecule for example dna, rna, different proteins, carbohydrates, fats etc.
 
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  • #2
divansaray said:
how to determine (theoretical, based on physics principles of detection of different fields from molecules without outside influence) individual electromagnetic field from single molecule for example dna, rna, different proteins, carbohydrates, fats etc.

If by electromagnetic field you mean light, then you can tag these molecules with a fluorophore and detect that as a surrogate.
 
  • #3
divansaray said:
how to determine (theoretical, based on physics principles of detection of different fields from molecules without outside influence) individual electromagnetic field from single molecule for example dna, rna, different proteins, carbohydrates, fats etc.

There are different types of optical spectroscopy. Absorption, photoluminescence, and Raman scattering are all useful ways to study these molecules. Optical spectroscopy works at visible, infrared and ultraviolet frequencies. Most of these spectroscopies work with far field effects, which means electromagnetic waves.

There are different spectroscopies that work at radio frequencies. Nuclear magnetic resonance and electron magnetic resonance use oscillating magnetic fields at radio frequencies. Most of these spectroscopies work under near field conditions. This means that the electromagnetic field in these spectroscopies are not true waves.

Electrochemical methods are also used to separate molecules when they are ionized. This is a bit of a static field measurement.
 
  • #4
I think X-ray crystallography? Or NMR but probably xray. it has to do with determining electron density. and there are a bunch of functions like coulombs law i think and something called the electrostatic constant that can be used that's needed for coulombs law to map isopotential lines.
 
  • #5
cimmerian said:
I think X-ray crystallography? Or NMR but probably xray. it has to do with determining electron density. and there are a bunch of functions like coulombs law i think and something called the electrostatic constant that can be used that's needed for coulombs law to map isopotential lines.
Xray crystallography can be used to determine the positions of atoms in the molecule, and the distribution of electrons around the nucleus of the atom. This is related to the "electromagnetic field" in the molecule.

There have been a lot of suggestions. The OP hasn't responded to any of them. It leads me to think that we don't know what he means by an "electromagnetic field". His concept of electromagnetic field may not be the same as what a scientist usually means by "electromagnetic field".

I think that we should wait with the suggestions until the OP gives us a better idea of what he is asking.
 

What is the purpose of determining single biomolecules?

The purpose of determining single biomolecules is to study and understand the characteristics and behavior of individual molecules in biological systems. This allows for a more detailed understanding of biological processes and diseases at a molecular level.

What techniques are commonly used for determining single biomolecules?

The most commonly used techniques for determining single biomolecules include fluorescence microscopy, atomic force microscopy, and single molecule spectroscopy. These techniques allow for the visualization and detection of individual molecules.

What are the challenges in determining single biomolecules?

One of the main challenges in determining single biomolecules is the low signal-to-noise ratio, as individual molecules emit very weak signals. Another challenge is the potential for photobleaching, where the fluorescence of molecules can fade over time.

How is single biomolecule detection used in research?

Single biomolecule detection is used in a variety of research fields, including genetics, biochemistry, and biomedicine. It allows for the study of individual molecules in complex biological systems, providing valuable insights into their functions and interactions.

What are the potential applications of single biomolecule detection?

The potential applications of single biomolecule detection include drug discovery, disease diagnosis, and personalized medicine. It can also be used in nanotechnology and biotechnology for the development of new materials and technologies based on single molecules.

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