Hydreogen Peroxide LAB

[mickeyminnie]

Analysis:
What are the effects of various temperature, pH, alcohol, and saline solution treatments on peroxidase activity?

What are the optional ranges of temperature and pH for peroxidase? Are alcohol and saline solution inhibitors or activators?

Sate the dependent, independent and controlled variables in this investigation

My answer:
Dependent- peroxidase (potato)
Independent – hydrogen peroxide
Controlled variable - distilled water

Why should hydrogen peroxide’s spntneous decomposition into gaseous oxygen be measured in the absence of any enzyme?

What is the significance of the other data be if this datum were not gathered?

Inquiry Skills:
Evlalute the procedure by identifying sources of error and suggesting any modifications to the method you designed that you feel would improve the accuracy and precision of the data.

Knowledge:
Question 5: Sate the optional range of temperature and pH for peroxidase.
Questions 6: The optional temperature and pH ranges of catalase are known to be 36-37 degree at a pH of 6.5 to 7.0. how does this profile compare with what you found for peroxidase in question#5?

I'm completely lost in this lab. I don't get proper help from my teacher since its a distance education course. Please someone help with this lab. I want HINTS

 

[Moonbear]

Without more details about your experiment, I can't really evaluate your answers at all to offer help.

I'm also assuming the answers are intended to be based on your actual data while conducting the lab. If you show some of that data, we might be able to help give you pointers.

 

[Blueshift5]

not answers.HINTS:

1. Effects of various treatments on peroxidase activity: To determine the effects of temperature, pH, alcohol, and saline solution treatments on peroxidase activity, you will need to perform experiments where you change one variable at a time while keeping the others constant. For example, you can test the activity of peroxidase at different temperatures (e.g. 10°C, 25°C, 40°C) while keeping the pH, alcohol, and saline solution concentrations constant. This will allow you to observe how each variable affects peroxidase activity.

2. Optional ranges of temperature and pH for peroxidase: The optimal temperature and pH for peroxidase may vary depending on the source of the enzyme (e.g. potato, liver, etc.). You can research the optimal ranges for peroxidase from different sources and compare them to see if there are any similarities or differences.

3. Alcohol and saline solution inhibitors or activators: Some substances, such as alcohol and saline solution, may act as inhibitors or activators of peroxidase activity. To determine their effects, you can perform experiments where you add different concentrations of alcohol or saline solution to the reaction mixture and measure the peroxidase activity.

4. Dependent, independent, and controlled variables: The dependent variable is the factor that is being measured or observed in the experiment (e.g. peroxidase activity). The independent variable is the factor that is being manipulated or changed (e.g. temperature, pH, alcohol, saline solution). The controlled variables are the factors that are kept constant throughout the experiment (e.g. amount of enzyme, concentration of substrate).

5. Measurement of hydrogen peroxide decomposition: Hydrogen peroxide can spontaneously decompose into water and oxygen, but the rate of this reaction can be increased by the presence of an enzyme such as peroxidase. Therefore, measuring the rate of decomposition in the absence of any enzyme can serve as a control to compare to the rate in the presence of peroxidase.

6. Significance of other data: Gathering data on the effects of different treatments on peroxidase activity is important because it can provide insight into how the enzyme functions under different conditions. This information can be used to understand the optimal conditions for peroxidase activity and how it may be affected by external factors.

7. Sources of error and modifications to improve accuracy and precision: Some sources of error in this experiment could include variations in enzyme and substrate