Transformation of bacterial cell

In summary, the conversation is about calculating the transformation efficiency and the formula for it. One person suggests a website to try and another person shares their formula, but is unsure about finding the amount of DNA plated. They mention that the transformation efficiency should be a large number, but their experiment did not go well.
  • #1
higherme
129
0
does anyone know how to calculate the Transformation efficiency?
 
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  • #3
actually I kind of have the formula of the transformation efficiency

colonies on plate/ amount of DNA plated in (ug)

I just don't know how to find the amounf of DNA plated... well i kind of have an answer
but it's really small... but this website saids that the the transformation efficiency should be a big number... something like 10^9 that they got from their example...

so, i don't know if i am doing it right...
 
  • #4
or maybe it's because my experient didn't work out that great
 

1. What is bacterial cell transformation?

Bacterial cell transformation is the process of introducing foreign DNA into a bacterial cell, resulting in a genetically modified organism. This can occur naturally or be artificially induced in a laboratory setting.

2. What is the purpose of bacterial cell transformation?

The purpose of bacterial cell transformation is to create genetically modified bacteria for various applications, such as producing proteins, antibiotics, or studying gene function.

3. How is bacterial cell transformation performed?

Bacterial cell transformation is typically performed by exposing bacterial cells to a specific chemical treatment or electric shock that creates small pores in the cell membrane. Foreign DNA is then introduced into the cells, and some of the cells will take up and incorporate this DNA into their own genome.

4. What types of bacteria can undergo transformation?

Most types of bacteria can undergo transformation, including both gram-positive and gram-negative bacteria. Some bacteria, such as Streptococcus pneumoniae, are naturally competent (able to take up foreign DNA) while others may require chemical or electrical induction.

5. What are the potential risks and benefits of bacterial cell transformation?

The potential benefits of bacterial cell transformation include the creation of genetically modified bacteria for various applications in medicine, industry, and research. However, there are also potential risks, such as the spread of genetically modified bacteria into the environment and the potential for unintended consequences or unintended gene transfer to other organisms.

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