|Sep7-12, 08:05 AM||#1|
Which HPLC column to choose
first off all, I know this isnīt the best forum for my questions about HPLC columns, but unfortunately on other forums either people donīt answer or donīt know what they are talking about.
So here my question:
I have to separate enantiomer proteins with HPLC columns. I read it is import which filter paper I used before so Iīll post it,too: Whatman filter paper grade 43
So far so good.
my problem now is that I need contaminations about nearly zero percent. Therefore Iīm using at the moment a HPLC column of Chiral. but all the time I get peaks that shouldnīt be there. Would it be better to change the manufacturer to Waters or Agilent?
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|Sep20-12, 07:42 AM||#3|
Iīm working in a labor for now 5 years among others with HPLC columns. In my opinion Whatman filter paper grade 43 should be fine, cause itīs a quantitative filter paper. I think for separating enantiomer proteins chiral HPLC columns are the best choice, though chiral columns of Waters or Agilent would have less contamination, too. Did you regard that one enantiomer protein always gives two peaks in a chiral HPLC column and one in a normal one?
Perhaps you should write a little bit more about your experiment.
For comparing HPLC columns Iīll also send you a link with a HPLC column configurator:
Hope it will be helpfull, good luck
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