Dear allI work with a spectrophotometer

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In summary, when using the spectrophotometer "Carry 17D" with wavelengths below 300nm, there may be fluctuations in the transition spectrum. To solve this issue and obtain a clear spectrum, it is recommended to use a brighter source, a better detector, or optics/gratings with lower losses. Alternatively, measuring and averaging multiple spectra can also reduce noise, but this method is time-consuming.
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atish
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dear all
I work with a spectrophotometer "Carry 17D". below 300nm wavelenght the transition spectrom have lots of flactuation. how to solve the problem and how to provide a clear spectrom?
regards
 
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Often spectrometers will compute a baseline and then normalise spectra to that baseline. If the signal to noise ratio drops at shorter wavelengths, then normalised spectra will become progressively noisier at the shorter wavelengths.

To resolve this, you need either a brighter source, a better detector, or optics/gratings that have lower losses below 300 nm.

Measuring lots of spectra and averaging them can also reduce the noise, but this approach is time-consuming.

Claude.
 

1. What is a spectrophotometer?

A spectrophotometer is a scientific instrument used to measure the intensity of light at different wavelengths. It works by passing a beam of light through a sample and measuring the amount of light that is absorbed or transmitted by the sample.

2. How does a spectrophotometer work?

A spectrophotometer works by using a monochromator to separate white light into its component wavelengths, then passing the selected wavelength through a sample and measuring the amount of light that is absorbed or transmitted. This data is then used to create a graph or spectrum that shows the absorption or transmission of light at different wavelengths.

3. What is the purpose of using a spectrophotometer?

The main purpose of using a spectrophotometer is to measure the amount of light that is absorbed or transmitted by a sample. This can be used to determine the concentration of a substance in a solution, to identify unknown substances, and to monitor chemical reactions.

4. What types of samples can be measured with a spectrophotometer?

A spectrophotometer can measure a wide range of samples, including liquids, gases, and solids. It can also measure samples in various forms, such as solutions, suspensions, and powders.

5. How accurate are spectrophotometer measurements?

The accuracy of spectrophotometer measurements depends on various factors, such as the quality of the instrument, the type of sample being measured, and the experimental conditions. Generally, spectrophotometers have a high level of accuracy and precision, with most measurements having an error of less than 1%. However, it is important to carefully follow proper procedures and calibrate the instrument regularly to ensure accurate results.

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