Antibiotica in mammalian cells

[sotellme]

if my vector has an amphicilin and a neomycin selection markers. which one should i use for selection of my mammalian cells?

thanks.

 

[iansmith]

You should understant the site of attack of the antibiotic you mention. Ampicilin attacks the cell wall synthesis whereas neomycin attacks the ribosome.

Mammalian cell do not have cell wall. The answer is neomycin.

Also most eukarytics cell are resitance to concentration of antibiotics used for bacteria. High concentration are required for selection.

 

[ravenprp]

It ultimately depends on the specific needs and goals of your experiment. Both ampicillin and neomycin are commonly used selection markers in mammalian cells and have their own advantages and disadvantages. Ampicillin is a beta-lactam antibiotic that inhibits bacterial cell wall synthesis and is commonly used in bacterial selection. However, it can also be used in mammalian cells as they contain beta-lactamase enzymes that can inactivate ampicillin. Neomycin, on the other hand, is an aminoglycoside antibiotic that inhibits protein synthesis and is effective in both bacterial and mammalian cells. It is not inactivated by beta-lactamase and has a higher selection efficiency compared to ampicillin.

If you are working with bacterial cells and want to avoid any potential interference from beta-lactamase enzymes, then neomycin would be a better choice. However, if you are specifically working with mammalian cells and want a more efficient selection process, then neomycin would be the preferred option. Ultimately, it is important to carefully consider your experimental needs and choose the selection marker that best fits your specific goals.