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benndamann33
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If you're reading a graph of absorption coefficient(cm^-1) versus wavelength(nm), how can you dtermine if the solution is optically dilute? What about optically thick? Thanks
Ben
Ben
An absorption spectrum is a graphical representation of the amount of light absorbed by a substance at different wavelengths. It is created by passing light through a sample and measuring the intensity of light that passes through the sample at different wavelengths.
The absorption spectrum of a substance is determined by using a spectrophotometer, which measures the amount of light transmitted through a sample at different wavelengths. The data collected is then plotted on a graph to create the absorption spectrum.
Dilute solutions have a lower concentration of the substance being analyzed, while thick solutions have a higher concentration. In absorption spectroscopy, thicker solutions will absorb more light than dilute solutions, resulting in a higher absorbance value.
The concentration of a solution directly affects its absorption spectrum. As the concentration increases, the absorbance also increases. This is because a higher concentration of a substance will result in more molecules absorbing light at a given wavelength.
Absorption spectroscopy has many applications in scientific research and industry. It is commonly used in pharmaceuticals, environmental testing, and food and beverage analysis. It can also be used to determine the concentration of a substance in a solution and to identify unknown substances by comparing their absorption spectra to known substances.