Register to reply

Differences in tagging Gram-negative and positive bacteria?

Share this thread:
Skwrl
#1
Mar17-14, 07:56 AM
P: 6
Hi there,

Recently I've been reading about tagging bacteria with GFP, mCherry and other fluorescent proteins. The article I'm reading now (Lagendijk et at., 2010, Genetic tools for tagging Gram negative bacteria with mCherry...) has information on tagging Gram-negative bacteria, but it doesn't say anything about Gram-positive ones. Is the principle similar or is tagging with marker genes different when the bacteria are Gram-positive?

Thank you in advance!
Phys.Org News Partner Biology news on Phys.org
Neutering project curbed feral cat population
Pollen on birds shows feeding grounds
Researchers look at small RNA pathways in maize tassels
iansmith
#2
Mar18-14, 07:44 PM
Emeritus
Sci Advisor
PF Gold
iansmith's Avatar
P: 1,430
As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.
Evo
#3
Mar18-14, 09:13 PM
Mentor
Evo's Avatar
P: 26,528
Quote Quote by iansmith View Post
As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.
Hey Ian!! Good to see you back!

Skwrl
#4
Mar21-14, 05:35 PM
P: 6
Differences in tagging Gram-negative and positive bacteria?

Thanks for the help!


Register to reply

Related Discussions
Component vectors positive or negative and angles positive or negative Introductory Physics Homework 5
Gram negative bacteria and antibiotics? Biology 2
Gram negative bacteria and blood agar hemolysis Biology, Chemistry & Other Homework 2
Different antibiotics on Gram positive and Gram negative bacteria Biology 1
Comparing effects of antibiotics on Gram +ve and -ve bacteria Biology 8