## Q: Fluorescence reference

I am involved in developing a microscopy course, and I would like to
provide a suitable discussion of the physics of fluorescence to the
students (PhD-track biophysics). I am unaware of a review or tutorial
article that covers the physics of fluorescence and phosphorescence of
*molecules*. Note, I am not interested in Jablonski-type diagrams of
atomic absorption and emission. I would like a molecular-level
treatment, using modern quantum notation. Any suggestions are appreciated.

Andy

--
Andrew Resnick, Ph.D.
Department of Physiology and Biophysics
Case Western Reserve University

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 Something general perhaps, like http://www.biophysics.org/education/cathy_royer.pdf or http://www.olympusmicro.com/primer/r...tutorials.html On Jul 5, 3:41 pm, Andy Resnick wrote: > I am involved in developing a microscopy course, and I would like to > provide a suitable discussion of the physics of fluorescence to the > students (PhD-track biophysics). I am unaware of a review or tutorial > article that covers the physics of fluorescence and phosphorescence of > *molecules*. Note, I am not interested in Jablonski-type diagrams of > atomic absorption and emission. I would like a molecular-level > treatment, using modern quantum notation. Any suggestions are appreciated. > > Thanks in advance, > > Andy > > -- > Andrew Resnick, Ph.D. > Department of Physiology and Biophysics > Case Western Reserve University
 Something general perhaps, like http://www.biophysics.org/education/cathy_royer.pdf or http://www.olympusmicro.com/primer/r...tutorials.html On Jul 5, 3:41 pm, Andy Resnick wrote: > I am involved in developing a microscopy course, and I would like to > provide a suitable discussion of the physics of fluorescence to the > students (PhD-track biophysics). I am unaware of a review or tutorial > article that covers the physics of fluorescence and phosphorescence of > *molecules*. Note, I am not interested in Jablonski-type diagrams of > atomic absorption and emission. I would like a molecular-level > treatment, using modern quantum notation. Any suggestions are appreciated. > > Thanks in advance, > > Andy > > -- > Andrew Resnick, Ph.D. > Department of Physiology and Biophysics > Case Western Reserve University

## Q: Fluorescence reference

Something general perhaps, like
http://www.biophysics.org/education/cathy_royer.pdf
or http://www.olympusmicro.com/primer/r...tutorials.html

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

 Something general perhaps, like http://www.biophysics.org/education/cathy_royer.pdf or http://www.olympusmicro.com/primer/r...tutorials.html On Jul 5, 3:41 pm, Andy Resnick wrote: > I am involved in developing a microscopy course, and I would like to > provide a suitable discussion of the physics of fluorescence to the > students (PhD-track biophysics). I am unaware of a review or tutorial > article that covers the physics of fluorescence and phosphorescence of > *molecules*. Note, I am not interested in Jablonski-type diagrams of > atomic absorption and emission. I would like a molecular-level > treatment, using modern quantum notation. Any suggestions are appreciated. > > Thanks in advance, > > Andy > > -- > Andrew Resnick, Ph.D. > Department of Physiology and Biophysics > Case Western Reserve University
 Something general perhaps, like http://www.biophysics.org/education/cathy_royer.pdf or http://www.olympusmicro.com/primer/r...tutorials.html On Jul 5, 3:41 pm, Andy Resnick wrote: > I am involved in developing a microscopy course, and I would like to > provide a suitable discussion of the physics of fluorescence to the > students (PhD-track biophysics). I am unaware of a review or tutorial > article that covers the physics of fluorescence and phosphorescence of > *molecules*. Note, I am not interested in Jablonski-type diagrams of > atomic absorption and emission. I would like a molecular-level > treatment, using modern quantum notation. Any suggestions are appreciated. > > Thanks in advance, > > Andy > > -- > Andrew Resnick, Ph.D. > Department of Physiology and Biophysics > Case Western Reserve University
 Mike Jr. wrote: > Something general perhaps, like > http://www.biophysics.org/education/cathy_royer.pdf > or http://www.olympusmicro.com/primer/r...tutorials.html I am looking for something more quantitative- more physical chemistry, I suppose. I have Lakowicz's book (the new edition, it's very good), but I am looking for something more in depth on the specific treatment of fluorescent proteins and (semi-)large molecules like FITC, Fura-2, etc. where molecular states, rather than atmoic states, are involved. -- Andrew Resnick, Ph.D. Department of Physiology and Biophysics Case Western Reserve University
 "Andy Resnick" wrote in message news:f6jhsi$j13$1@eeyore.INS.cwru.edu... :I am involved in developing a microscopy course, and I would like to : provide a suitable discussion of the physics of fluorescence to the : students (PhD-track biophysics). I am unaware of a review or tutorial : article that covers the physics of fluorescence and phosphorescence of : *molecules*. Note, I am not interested in Jablonski-type diagrams of : atomic absorption and emission. I would like a molecular-level : treatment, using modern quantum notation. Any suggestions are appreciated. : : Thanks in advance, : : Andy : : : -- : Andrew Resnick, Ph.D. : Department of Physiology and Biophysics : Case Western Reserve University Do you mean bioluminescence, fluorescence or phosphorescence? bioluminescence: the emission of light from living organisms e.g. fireflies, (chartreuse = greenish yellow) http://www.lifesci.ucsb.edu/~biolum/ fluorescence: luminescence that is caused by the absorption of radiation at one wavelength followed by nearly immediate reradiation usually at a different wavelength and that ceases almost at once when the incident radiation stops. e.g. lasers (ruby red). phosphorescence: luminescence that is caused by the absorption of radiations (as light or electrons) and continues for a noticeable time after these radiations have stopped e.g. TV tube screens (red/green/blue phosphors). Persistence is important or flicker can result. Note: Changing the anode voltage changes the colour. As a secondary effect, more readily noticeable, it changes image size. http://www.patentstorm.us/patents/5336080-claims.html http://ieeexplore.ieee.org/Xplore/lo...0/01451112.pdf
 On Jul 6, 12:41 am, "Mike Jr." wrote: > Something general perhaps, likehttp://www.biophysics.org/education/cathy_royer.pdf > orhttp://www.olympusmicro.com/primer/resources/tutorials.html > > On Jul 5, 3:41 pm, Andy Resnick wrote: > > > I am involved in developing a microscopy course, and I would like to > > provide a suitable discussion of the physics of fluorescence to the > > students (PhD-track biophysics). I am unaware of a review or tutorial > > article that covers the physics of fluorescence and phosphorescence of > > *molecules*. Note, I am not interested in Jablonski-type diagrams of > > atomic absorption and emission. I would like a molecular-level > > treatment, using modern quantum notation. Any suggestions are appreciated. > > > Thanks in advance, > > > Andy > > > -- > > Andrew Resnick, Ph.D. > > Department of Physiology and Biophysics > > Case Western Reserve University Perhaps "Methods of single-molecule fluorescence spectroscopy and microscopy", W. E. Moerner and David P. Fromm Department of Chemistry, Stanford University, Mail Code 5080, Stanford, California 94305-5080 See: http://scitation.aip.org/getabs/serv...cvips&gifs=yes Also, "Annual Review of Biophysics and Biomolecular Structure" Vol. 32: 161-182 (Volume publication date June 2003) (doi:10.1146/annurev.biophys.32.110601.142525) First published online as a Review in Advance on February 11, 2003 "THE POWER AND PROSPECTS OF FLUORESCENCE MICROSCOPIES AND SPECTROSCOPIES" Xavier Michalet,1 * Achillefs N. Kapanidis,1 * Ted Laurence,1 * Fabien Pinaud,1 * Soeren Doose,1 * Malte Pflughoefft,1 and * Shimon Weiss1,2 * 1Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E. Young Drive East, Los Angeles, California 90095; 2Department of Physiology, UCLA, Young Hall, 607 Charles E. Young Drive East, Los Angeles, California 90095; See: http://arjournals.annualreviews.org/...alCode=biophys
 On Jul 5, 3:41 pm, Andy Resnick wrote: > I am involved in developing a microscopy course, and I would like to > provide a suitable discussion of the physics of fluorescence to the > students (PhD-track biophysics). I am unaware of a review or tutorial > article that covers the physics of fluorescence and phosphorescence of > *molecules*. Note, I am not interested in Jablonski-type diagrams of > atomic absorption and emission. I would like a molecular-level > treatment, using modern quantum notation. Any suggestions are appreciated. > > Thanks in advance, > > Andy > > -- > Andrew Resnick, Ph.D. > Department of Physiology and Biophysics > Case Western Reserve University A good review article, with plenty of applications: "Photophysics and Mechanisms of Intramolecular Electronic Energy Transfer in Bichromophoric Molecular Systems: Solution and Supersonic Jet Studies" Chem. Rev. 1996, 96 (6), 1953 -1976.
 On Jul 5, 3:41 pm, Andy Resnick wrote: > I am involved in developing a microscopy course, and I would like to > provide a suitable discussion of the physics of fluorescence to the > students (PhD-track biophysics). I am unaware of a review or tutorial > article that covers the physics of fluorescence and phosphorescence of > *molecules*. Note, I am not interested in Jablonski-type diagrams of > atomic absorption and emission. I would like a molecular-level > treatment, using modern quantum notation. Any suggestions are appreciated. > > Thanks in advance, > > Andy > > -- > Andrew Resnick, Ph.D. > Department of Physiology and Biophysics > Case Western Reserve University This is a more specific review, on Forster resonance energy transfer, which has great application in biology. Chem. Rev., 96 (6), 1953 -1976, 1996 It has plenty of hard-core QM, and references galore.

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