Q: Fluorescence reference

Andy Resnick

I am involved in developing a microscopy course, and I would like to
provide a suitable discussion of the physics of fluorescence to the
students (PhD-track biophysics). I am unaware of a review or tutorial
article that covers the physics of fluorescence and phosphorescence of
*molecules*. Note, I am not interested in Jablonski-type diagrams of
atomic absorption and emission. I would like a molecular-level
treatment, using modern quantum notation. Any suggestions are appreciated.

Thanks in advance,

Andy


--
Andrew Resnick, Ph.D.
Department of Physiology and Biophysics
Case Western Reserve University

Mike Jr.

Something general perhaps, like
http://www.biophysics.org/education/cathy_royer.pdf
or http://www.olympusmicro.com/primer/r...tutorials.html

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

Mike Jr.

Something general perhaps, like
http://www.biophysics.org/education/cathy_royer.pdf
or http://www.olympusmicro.com/primer/r...tutorials.html

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

Mike Jr.

Something general perhaps, like
http://www.biophysics.org/education/cathy_royer.pdf
or http://www.olympusmicro.com/primer/r...tutorials.html

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

Mike Jr.

Something general perhaps, like
http://www.biophysics.org/education/cathy_royer.pdf
or http://www.olympusmicro.com/primer/r...tutorials.html

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

Mike Jr.

Something general perhaps, like
http://www.biophysics.org/education/cathy_royer.pdf
or http://www.olympusmicro.com/primer/r...tutorials.html

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

Andy Resnick

Mike Jr. wrote:
> Something general perhaps, like
> http://www.biophysics.org/education/cathy_royer.pdf
> or http://www.olympusmicro.com/primer/r...tutorials.html

I am looking for something more quantitative- more physical chemistry, I
suppose. I have Lakowicz's book (the new edition, it's very good), but I
am looking for something more in depth on the specific treatment of
fluorescent proteins and (semi-)large molecules like FITC, Fura-2, etc.
where molecular states, rather than atmoic states, are involved.


--
Andrew Resnick, Ph.D.
Department of Physiology and Biophysics
Case Western Reserve University

Androcles

"Andy Resnick" <andy.resnick@op.case.edu> wrote in message
news:f6jhsi$j13$1@eeyore.INS.cwru.edu...
:I am involved in developing a microscopy course, and I would like to
: provide a suitable discussion of the physics of fluorescence to the
: students (PhD-track biophysics). I am unaware of a review or tutorial
: article that covers the physics of fluorescence and phosphorescence of
: *molecules*. Note, I am not interested in Jablonski-type diagrams of
: atomic absorption and emission. I would like a molecular-level
: treatment, using modern quantum notation. Any suggestions are
appreciated.
:
: Thanks in advance,
:
: Andy
:
:
: --
: Andrew Resnick, Ph.D.
: Department of Physiology and Biophysics
: Case Western Reserve University

Do you mean bioluminescence, fluorescence or phosphorescence?

bioluminescence:
the emission of light from living organisms

e.g. fireflies, (chartreuse = greenish yellow)
http://www.lifesci.ucsb.edu/~biolum/



fluorescence:
luminescence that is caused by the absorption of radiation at one
wavelength followed by nearly immediate reradiation usually at a different
wavelength and that ceases almost at once when the incident radiation stops.

e.g. lasers (ruby red).


phosphorescence:
luminescence that is caused by the absorption of radiations (as light or
electrons) and continues for a noticeable time after these radiations have
stopped

e.g. TV tube screens (red/green/blue phosphors).
Persistence is important or flicker can result.

Note: Changing the anode voltage changes the colour. As a secondary
effect, more readily noticeable, it changes image size.
http://www.patentstorm.us/patents/5336080-claims.html
http://ieeexplore.ieee.org/Xplore/lo...0/01451112.pdf

Mike Jr.

On Jul 6, 12:41 am, "Mike Jr." <n00s...@comcast.net> wrote:
> Something general perhaps, likehttp://www.biophysics.org/education/cathy_royer.pdf
> orhttp://www.olympusmicro.com/primer/resources/tutorials.html
>
> On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
>
> > I am involved in developing a microscopy course, and I would like to
> > provide a suitable discussion of the physics of fluorescence to the
> > students (PhD-track biophysics). I am unaware of a review or tutorial
> > article that covers the physics of fluorescence and phosphorescence of
> > *molecules*. Note, I am not interested in Jablonski-type diagrams of
> > atomic absorption and emission. I would like a molecular-level
> > treatment, using modern quantum notation. Any suggestions are appreciated.
>
> > Thanks in advance,
>
> > Andy
>
> > --
> > Andrew Resnick, Ph.D.
> > Department of Physiology and Biophysics
> > Case Western Reserve University

Perhaps "Methods of single-molecule fluorescence spectroscopy and
microscopy",
W. E. Moerner and David P. Fromm
Department of Chemistry, Stanford University, Mail Code 5080,
Stanford, California 94305-5080

See: http://scitation.aip.org/getabs/serv...cvips&gifs=yes

Also, "Annual Review of Biophysics and Biomolecular Structure"
Vol. 32: 161-182 (Volume publication date June 2003)
(doi:10.1146/annurev.biophys.32.110601.142525)
First published online as a Review in Advance on February 11, 2003

"THE POWER AND PROSPECTS OF FLUORESCENCE MICROSCOPIES AND
SPECTROSCOPIES"

Xavier Michalet,1 * Achillefs N. Kapanidis,1 * Ted Laurence,1 * Fabien
Pinaud,1 * Soeren Doose,1 * Malte Pflughoefft,1 and * Shimon Weiss1,2
*

1Department of Chemistry and Biochemistry, UCLA, Young Hall, 607
Charles E. Young Drive East, Los Angeles, California 90095;

2Department of Physiology, UCLA, Young Hall, 607 Charles E. Young
Drive East, Los Angeles, California 90095;

See: http://arjournals.annualreviews.org/...alCode=biophys

The_Man

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

A good review article, with plenty of applications:

"Photophysics and Mechanisms of Intramolecular Electronic Energy
Transfer in Bichromophoric Molecular Systems: Solution and Supersonic
Jet Studies"

Chem. Rev. 1996, 96 (6), 1953 -1976.

The_Man

On Jul 5, 3:41 pm, Andy Resnick <andy.resn...@op.case.edu> wrote:
> I am involved in developing a microscopy course, and I would like to
> provide a suitable discussion of the physics of fluorescence to the
> students (PhD-track biophysics). I am unaware of a review or tutorial
> article that covers the physics of fluorescence and phosphorescence of
> *molecules*. Note, I am not interested in Jablonski-type diagrams of
> atomic absorption and emission. I would like a molecular-level
> treatment, using modern quantum notation. Any suggestions are appreciated.
>
> Thanks in advance,
>
> Andy
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> Case Western Reserve University

This is a more specific review, on Forster resonance energy transfer,
which has great application in biology.

Chem. Rev., 96 (6), 1953 -1976, 1996

It has plenty of hard-core QM, and references galore.