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Determine amount of glucose-1-phosphate & glucose-1,6-diphosphate
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Mar31-08, 08:11 PM
1. The problem statement, all variables and given/known data
In a mixture of glucose-1-phosphate and glucose-1,6-diphosphate, how could you determine the amount of each in the mixture?
2. Relevant equations
3. The attempt at a solution
I was thinking of using a strong acid to separate the phosphate from the glucose and use Fiske-Subbarow assay to determine the amount of glucose-1-phosphate, but won't this also affect the glucose-1,6-diphosphate? I am having trouble figuring out how to determine the amount of glucose-1,6-diphosphate in the mixture.
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