|Jun11-08, 03:53 PM||#1|
Genetics: Bacterial Genetics and Specialized Transduction
1. The problem statement, all variables and given/known data
Compare and contrast the transfer of the F factor and the lambda phage during conjugation under the following conditions:
a) Hfr([tex]\lambda[/tex]) x F-
b) Hfr x F- ([tex]\lambda[/tex])
c) Hfr([tex]\lambda[/tex]) x F-([tex]\lambda[/tex])
3. The attempt at a solution
a) Hfr infects F- which is non-immune --> lysis occurs
c) Hfr tries to infect F- with lambda already inserted: immune---> no lysis
The transfer where F- already has lambda inserted I'm not sure of what happens. The other two are textbook. My guess would be that Hfr infects F- and excises phage lambda out of the F- which would be non-immune but I don't think lysis would occur.
Any help or guidance would be appreciated.
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