# Beer-Lambert equation

by nobahar
Tags: beerlambert, equation
 P: 501 1. The problem statement, all variables and given/known data I need to use the Lambert-Beer equation to plot optical density of a lactate solution. 2. Relevant equations $$A=\varepsilon\ell C$$ (The path length is 1 cm) 3. The attempt at a solution This is one of my four samples (if you can show me where I'm going wrong with this then I can apply the correction to the others): 2.22 $$\mu$$mol in 1.5ml, therefore there is 0.296 $$\mu$$mol in 0.2ml This info seems to match to the example graph I have. But using the formula above, where $$\varepsilon$$=6.22 for micromol/ml, I get: 6.22 x (0.296/0.2) micromol/ml = 9.2056 This doesn't match up with the graph I have! Any help appreciated! Please!