The limitations of RP-HPLC


by Shaybay92
Tags: chemical analysis, hplc, limitations, organic chemistry, rp-hplc
Shaybay92
Shaybay92 is offline
#1
Aug29-09, 05:49 AM
P: 122
As HPLC is such a common method in chemical analysis, I was just wondering what are the limitations to such a technique? What compounds/mixtures is it limited to analyzing?
Phys.Org News Partner Chemistry news on Phys.org
Repeated self-healing now possible in composite materials
Potent, puzzling and (now less) toxic: Team discovers how antifungal drug works
Research offers 'promise' of improved food safety
chemisttree
chemisttree is offline
#2
Sep5-09, 09:16 AM
Sci Advisor
HW Helper
PF Gold
chemisttree's Avatar
P: 3,725
Well it certainly is limited to the analysis of compounds which are soluble in the mobile phase. It is limited to the analysis of compounds which are not reactive with the mobile phase or stationary phase as well.


Register to reply

Related Discussions
Waters 440 Absorbance Detector for HPLC? Electrical Engineering 2
Helium Source in HPLC Chemistry 1
[SOLVED] Chiral Purity (enantiomer excess) for HPLC Chemistry 3
The limitations of Lenz law? Introductory Physics Homework 0
interactions with the mobile phase Chemistry 2