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Bradford Method Lab

by lha08
Tags: bradford, method
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Mar24-10, 08:40 PM
P: 164
1. The problem statement, all variables and given/known data
I did a lab which consisted of using the Bradford method and graphed the standard protein concentration and the absorbancies of BSA standard dilutions and used the slope in order to determine the protein concentrations of 3 unknown samples using their absorbance values. When I found the protein concentrations of the 3 unknown samples (which are milk, protein drink and soy milk) and compared their experimental values to their actual protein concentration on the nutritional label, they were significantly lower. Like for example, the experimental protein conc. for the milk i got 21576 µg/ml, while the value on the label is 36000 µg/ml...I'm having a hard time finding out why they are lower? Is it because of errors in the experiment?
I don't know if I gave enough detail of the lab so if there's anything you don't understand, please tell me.

2. Relevant equations

3. The attempt at a solution
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