negative absorbance reaading

rick112

hi

i know this is kind of a basic question..but what in reality does a negative absorbance reading means?? why cant the spectrometer just say its out the reading range or something??
does a negative reading really say something can we look at it and detect something...like if the negative reading is x that means it has so much of some component...

johng23

This sounds like a question for the spectrometer's manual perhaps...?

Sybren

that means your measurement is not well calibrated. a negative absorption from an excited sample would indicate stimulated emission

Galap

It seems like the spectrometer needs to be calibrated. It's like pushing the zero or tare on a balance. You manually set which point is called zero and the device reads other values with respect to that.

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rick112	negative absorbance reaading Tweet hi i know this is kind of a basic question..but what in reality does a negative absorbance reading means?? why cant the spectrometer just say its out the reading range or something?? does a negative reading really say something can we look at it and detect something...like if the negative reading is x that means it has so much of some component...

johng23	This sounds like a question for the spectrometer's manual perhaps...?

Sybren	that means your measurement is not well calibrated. a negative absorption from an excited sample would indicate stimulated emission

Galap	negative absorbance reaading It seems like the spectrometer needs to be calibrated. It's like pushing the zero or tare on a balance. You manually set which point is called zero and the device reads other values with respect to that.