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Negative absorbance reaading

by rick112
Tags: absorbance, negative, reaading
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rick112
#1
Jul10-10, 02:24 PM
P: 7
hi

i know this is kind of a basic question..but what in reality does a negative absorbance reading means?? why cant the spectrometer just say its out the reading range or something??
does a negative reading really say something can we look at it and detect something...like if the negative reading is x that means it has so much of some component...
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johng23
#2
Jul10-10, 03:13 PM
P: 292
This sounds like a question for the spectrometer's manual perhaps...?
Sybren
#3
Jul10-10, 03:27 PM
P: 47
that means your measurement is not well calibrated. a negative absorption from an excited sample would indicate stimulated emission

Galap
#4
Jul10-10, 11:32 PM
P: 137
Negative absorbance reaading

It seems like the spectrometer needs to be calibrated. It's like pushing the zero or tare on a balance. You manually set which point is called zero and the device reads other values with respect to that.


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