Register to reply

Mixed Culture 16s rRNA

by HOMO
Tags: 16s ribosomal rna, 16s rrna
Share this thread:
HOMO
#1
Nov1-13, 11:45 PM
P: 4
I have a mixed culture plate and I want to do 16s rRNA analysis on it and I have a few questions.

First of all there are no visible colonies, but I know bacteria is growing. Do I just take a small chunk of the medium and do PCR on it with universal primers (I have a procedure for this step)?

Second, because it is mixed culture, wont the PCR be an amplification of a bunch of different 16s from the different bacteria? How do I separate the strains? I have an idea how to do this. After PCR on the universal primers, I can run it out on a gel and excise the separate fragments, then purify so that I have pure samples of all the amplified RNA?

Third, after I purify the RNA, I send it to get sequenced and then I use some database such as BLAST to see what I have?
Phys.Org News Partner Biology news on Phys.org
Rising temperatures can be hard on dogs
It takes two to court: Researchers identify functions of two classes of mouse pheromone receptors
Underground amphibians evolved unique ear

Register to reply

Related Discussions
16S rRNA sequence information to identify organisms Biology 1
Albertros culture Social Sciences 5
Could the culture war become civil war? Current Events 98
Our culture, Gen X General Discussion 3