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Glow in the dark E.Coli

by Warpspeed13
Tags: dark, ecoli, glow
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Warpspeed13
#1
Sep1-14, 09:11 PM
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Would the methods used to create glow in the dark e.coli readily work on other bacteria?
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Simon Bridge
#2
Sep2-14, 07:09 AM
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http://www.newscientist.com/article/...l#.VAWzUlR53t4
Warpspeed13
#3
Sep2-14, 08:11 AM
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Ok I guess I'll be more specific.What I mean is will the high school lab experiment method of freezing them and then heat shocking them readily work on other bacteria. Sorry for the unspecific first question I was tired and worded it vaguely.

Simon Bridge
#4
Sep2-14, 08:22 AM
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Glow in the dark E.Coli

What no plasmid? - do you have a reference?

All the ones I've seen are like this:
http://grad.bio.uci.edu/ecoevo/ahebl...ngBacteria.pdf

... and that is pretty much what the New Scientist article is talking about.
Warpspeed13
#5
Sep2-14, 08:42 AM
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Quote Quote by Simon Bridge View Post
What no plasmid? - do you have a reference?



All the ones I've seen are like this:

http://grad.bio.uci.edu/ecoevo/ahebl...ngBacteria.pdf



... and that is pretty much what the New Scientist article is talking about.

Here's the procedure http://www.apsnet.org/edcenter/K-12/...iBacterium.pdf
Warpspeed13
#6
Sep2-14, 08:42 AM
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I've done it myself in high school.
Warpspeed13
#7
Sep2-14, 08:45 AM
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Yep same procedure will it readily transfer to other bacteria?
Ryan_m_b
#8
Sep2-14, 01:08 PM
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GFP (and its derivatives of different colours) has been successfully expressed by multiple species of bacteria. It's even been used in fish and mammals.
Warpspeed13
#9
Sep2-14, 01:27 PM
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Do you know where the derivative Dolores can be purchased? I want to take pond scum from a local lake and make a glowing variety for a freshwater biome
Warpspeed13
#10
Sep2-14, 01:28 PM
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In a fish tank
Ryan_m_b
#11
Sep2-14, 01:36 PM
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Please provide a link to "Dolores" so we can know exactly what it is you are asking. Note that GFP shines in UV light so you'll have to have a UV lamp to see anything.
Warpspeed13
#12
Sep2-14, 01:37 PM
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Uggg stupid autocorrect. Sorry for the hassle I meant colors.
Ryan_m_b
#13
Sep2-14, 01:40 PM
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I know you can get kits from here:

http://www.bio-rad.com/en-uk/categor...asmid-gfp-kits

Not sure if they can be bought by a member of public though, or what colours they sell.
Ygggdrasil
#14
Sep2-14, 02:18 PM
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Quote Quote by Warpspeed13 View Post
Do you know where the derivative Dolores can be purchased? I want to take pond scum from a local lake and make a glowing variety for a freshwater biome
Note that most of the organisms commonly referred to as pond scum are not bacteria, but algae, a diverse group of unicellular eukaryotic organisms. While it is certainly possible to introduce fluorescent transgenes into these organisms, the procedures may be different from those used to modify bacteria. However, because there has been considerable research into modifying algae for biofuel applications, there may be some published, standard procedures for introducing transgenes into algae.
Warpspeed13
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Sep2-14, 05:43 PM
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Quote Quote by Ygggdrasil View Post
Note that most of the organisms commonly referred to as pond scum are not bacteria, but algae, a diverse group of unicellular eukaryotic organisms. While it is certainly possible to introduce fluorescent transgenes into these organisms, the procedures may be different from those used to modify bacteria. However, because there has been considerable research into modifying algae for biofuel applications, there may be some published, standard procedures for introducing transgenes into algae.

Cool thanks, are you by any chance on the bungie forums?
Simon Bridge
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Sep3-14, 02:19 AM
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From your reference:
In this lab, Green Fluorescent Protein (GFP) from the bioluminescent jellyfish Aequorea victoria has been incorporated into a plasmid along with a gene for resistance to the antibiotic ampicillin...
... which is the procedure referred to in the New Scientist article in post #2 isn't it?

Short answer "yes".
Ygggdrasil
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Sep3-14, 12:49 PM
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Quote Quote by Warpspeed13 View Post
Cool thanks, are you by any chance on the bungie forums?
Nope, sorry.
Warpspeed13
#18
Sep3-14, 12:50 PM
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Kk,.


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