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    Introduction to optics

    Dear, I want to know about a book explaining easily the fundamentals of optics(not geometric). I'm not a student who studys physics or optics but grad student studying laser spectroscopy(single molecule fluorescence spectroscopy). Are there any books matching me? Thank you.
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    Diffraction limited spot

    Thank you. Then what's the factor to improve the limited spot I can see in terms of NA or focal lenth?
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    Diffraction limited spot

    Dear everyone, I'm wondering what the diffraction limited spot is. When a laser(CW) is used to excite a fluorescent single molecule, the fluorescence from a single molecule is shown in the CCD camera. The size of fluorescence molecule is determined by Airy Disc which comes from diffraction...
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    Gaussian Beam Radius

    I'm doing FRAP(fluorescence recovery after photobleaching) Diffusion coefficient is what I wanna calculate. t=w^2*f/(4D) t:halftime of recovery fluorescence w:radius of the focused circular laser beam at the e^(-2) intensity f : correction factor for the amount of bleaching D:diffusion...