I want to know about a book explaining easily the fundamentals of optics(not geometric).
I'm not a student who studys physics or optics but grad student studying laser spectroscopy(single molecule fluorescence spectroscopy). Are there any books matching me?
I'm wondering what the diffraction limited spot is.
When a laser(CW) is used to excite a fluorescent single molecule, the fluorescence from a single molecule is shown in the CCD camera. The size of fluorescence molecule is determined by Airy Disc which comes from diffraction...
I'm doing FRAP(fluorescence recovery after photobleaching)
Diffusion coefficient is what I wanna calculate.
t:halftime of recovery fluorescence
w:radius of the focused circular laser beam at the e^(-2) intensity
f : correction factor for the amount of bleaching