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  1. chem_tr

    Lysis buffer

    Well, I appreciate your tries to fully understand the process. You are doing the correct one. As you know, Tris is a buffer with changeable pH values. If you add some EDTA (disodium salt, I suppose), the pH value slightly shifts to the alkaline side. It is possible that they noted the final...
  2. chem_tr

    So confused!

    Hello Please remember that some protocols do not reveal some data in order to maintain full usage. Your approach is a logical one, so I conclude that there is something not disclosed. 10 microliters of proteinase K with a concentration of 20 mg/mL makes 10 micrograms of enzyme solution. I...