Well, your not alone! ( :
I was going to post the steps before but i thought it would be to much. But, I'm post them so you can have a look see...Line 16 through 18 get to the heart of what i need to find. Trying to find what volume to multiple by is a bit tricky too. Mixed and diluted the...
Thanks for the mu: µ,
Let me see if I understand this correctly. if the acid enzyme is 1.022 times the pure enzyme concentration of 1mg/mL, that would give 1.022 x 1mg/mL = 1.022 mg/mL correct? I believe the steeper the slope for the extract indicates that I extracted more...
Maybe if i show you what i did it might help.
Extraction and Quantification of Acid Phosphatase Enzyme from Wheat
1. Obtain a 15 mL conical centrifugation tube and add 0.5 grams of raw
wheat germ to the tube.
2. Add 5 mL of 0.05M Sodium Acetate buffer, pH 4.5. Cap and shake the...
The pure anzyme was given at a 1mg/mL concentration.
The question is: How much (ug) of acid phosphatase enzyme was extracted from the wheat germ?
I believe the 1.022 is in (mg/mL). Dont I need to multiply this by a volume to get to a mass?
Or is this the mass?
I'm creating a Michealis/Menten curve and need to find the X values for the graph.
These are the instructions given to find the X values:
To obtain the X value, plot the molar concentration (molarity) of substrate used in each well...
One more thing...This might be a stupid question. But once you've found I1 and I2. If one of those is a negative number...it indicates that the current is flowing in the other direction. So, if I3 = I1 + I2 and let’s say that I1=-1, and I2 = 1would that change the equation to
I3= I1-I2 giving...
I'm not sure what to do when i hit a battery. I know that you assign a positive value to the battery if the battery output follows the tracing direction. But, for some reason it dosnt always work out. Like you see in this problem....
I’m having some trouble with Kirchhoff's circuit law problems regarding two batteries in the circuit. I've attached a crude picture of a problem i picked to understand whats going on.
So for the bottom loop I have
5V - 5v = i1 - i2
0= i1 – i2
The top loop I’m coming up with
5 = i2...
OK...heres what I've done. Flipped the triangle a little setting C and B on the x axis. I'm new to all this stuff so please forgive my ignorance.
Fbc = 691.2
Fac = 74.72
Fbcx = 691.2
Fbcy = 0
Facx = 74.72N cos(80) = 12.28
Facy = 74.72 sin(80) = 73.58
Fbcx + Fbcy
691.2 + 12.28 =...
Hi, Im working on the following question.
A 64 µC charge is located 30cm to the left of a 16 µC charge. What is the resultant force on a -12 µC charge positioned exactly 50mm below the 16 µC charge?
The Attempt at a...