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http://cancerweb.ncl.ac.uk/cgi-bin/omd?cpg+islands [Broken]

http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/manip/conc.html [Broken] I like isopropanol instead of ethanol, but that is more of a personal preference thing. Good luck.

Common?...I'm not sure, but beta-lactam antibiotics in the context of a serious bacterial infection might qualify, as there are so many people allergic to penicillin.

Monique, I haven't been to the lab in awhile, as I am graduated and taking a little time off, but that IS a good idea. I am a good 40 minutes from the lab however. Thank you so much for your help Ian and Monique. I do appreciate it.

Electrically operated (either by battery or plug in), and uses vacuum to direct fluid transfer into and out of pipettes that you attach to it. I always thought they were automated pipettors but I can't find anything like what I use in the lab on the web. Any thoughts? I'm looking to purchase...

Well anytime you leave any sort of sugar source out at room temp, you up the odds of having an escalation of the resident microbial population. However given the amount of time you are talking, the reason it is tought is because there is also evaporation at work. Try this. Get the same...

In a word...evaporation.

For those of you with the Alberts text... Page 999, top paragraph: "Once the pre-RC has been assembled in G1, the replication origin is ready to fire . The activation of S-Cdk in late G1 pulls the trigger and initiates DNA replcation. The initiation of replication also requires the...

I don't know if I can effectively convey what I am doing here, but I am going to try because I need the help. I am trying to determine my ratio of acid to base and subsequently make a .5M Tris-HCl buffer utilizing Tris base, and concentrated HCl. By my calculations I get a 19.05 parts acid/1...

I am looking for information regarding bovine or (preferrably) horse hemoglobin (human may even work). Specifically, I would like to know molecular weight, heat stability characteristics and behavior in any popular detergents (SDS would be nice). The link below was the only thing I turned up...

I am starting to make the various buffers for my research, but I am a bit rusty with my chemistry. I want to know if hendersen hasselbach equation can be used if the desired pH is higher than the pKA. Specifically, I want to make 1.5 M Tris-HCl buffer (pH 8.8) utilizing Tris base and HCl. I...

I just asked this question in my Mycology class. Fungal walls are either (usually) composed of chitin or cross-links of N-Acetylglucosamine. Either way, they don't seem to pose a problem for nutrient uptake as they go right through the cell wall. I think the primary purpose of the cell wall...

Aren't there drugs available to those that have been exposed in a 12-24 hour time-frame that dramatically lowers the odds of being infected?

Nevermind, I think I figured it out.

Is phylum=division? Thanks!

That does look good. If you don't get it let me know.

What is the difference exactly? Thanks!

OK. Found it...it was in the Lewin text, I have been using Alberts as a secondary source, they are both excellent. It looks to me like it is in the context of protein expression, but why don't you be the judge: "The metallothionein (MT) gene provides an example of how a single gene may be...

Uh oh...It was a long night and I can't remember if that was the Alberts or the Lewin text. I'll get it posted in a little bit.

"expressed at a basal level"; used in reference to expression of the the human metallothionein gene. Any thoughts would be most welcome. Thanks!

Perhaps this will be of use to other people as well. I am looking for a book or books that basically consists of techniques used and currently in use in the field of biochemistry and molecular biology with an emphasis on theory and NOT protocol. I will start, as I do have one good one, but...

I am a bit confused I guess about exactly what these are. If anyone could offer up an example of each, I would be very grateful. I did find this much: http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=cis+acting [Broken]

Well Another God, it looks like you are going to get your wish! What is a nucleosome positioning element?

Looks like that was all I could find as well. Maybe you could post an easier question? http://www.accelrys.com/cases/images/melanin3.jpg [Broken] http://www.accelrys.com/cases/images/melanin2.jpg [Broken]

Well I didn't find anything regarding taste.

http://pregnancytoday.com/resource/definitions/amniofluid.htm http://www.marchofdimes.com/professionals/681_4536.asp http://babies.sutterhealth.org/laboranddelivery/ld_am-flu.html Looks like a lot of this stuff depends on who you consult with. This should give yo a start though.

Well, I wasn't aware that any necessary readings measured by the computer were taken from the tach. Unless I am mistaken, the amount of fuel metered by the ECM for the engine is based on MAF, TPS,, CTS, and IAT/MAT readings. In your scenario, the engine would still be pumping air to some...

Basically just the part about the engine not needing any fuel at all. There are a lot of reasons I disagree, but I will just name one for now. What you are describing is essentially "displacement on demand". A neat concept developed in the mid-late '70's by Cadillac in which the theory...

The amount of gas you burn at any given time depends on a lot of factors, but only (ultimately) one you can control while you are in the cabin (unless you are using a direct link to a fuel injected car with aftermarket software)is the throttle position. GENERALLY speaking, driving faster versus...

If you do a google search with moblity shift essay you will find plenty of protocols and explanation. I did do a google search, but the results of my find were pretty protocol intensive, and my focus was on theory. I also like to believe that depending on the subject material, some members...

I am looking for some good links on this particular methodology. Anybody have any?

My answer to this would be that every organism I can think of relies on a protein-protein, and protein-DNA interactions as a fundamental basis for the sustainance (sp??) of life. Assuming for a moment that you could get a viable fetus (I have never had developmental or embryology mind you), it...

facial swelling, rashes, swelling that affects breathing, abnormal sensitivity to sking irritation; etc. Benadryl slows/ stops all this temporarily.

That link you posted isn't working?

Are you asking what an allergic reaction is or what specifically alllergic reactions she is having?

Looks to me like they are very close to being the same thing? http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=arteriosclerosis [Broken] http://cancerweb.ncl.ac.uk/cgi-bin/omd?atherosclerosis [Broken]

I have a friend who has developed some really horrendous allergies lately, and it is my suspicion that it is because she is a heavy smoker (there is a correlation between when she started and the allergies). I can't find anything specific in my immuno textbook though. Can anyone refer me to...

I second this idea. I would also recommend a subscription to a general biology journal or magazine of some sort. If the topic really interests you, and you read all your issues, you would be amazed how much you actually pick up.

You are right. I don't know what I was thinking...brain lapse I guess.[zz)] Ian pretty much hit on the head with the core promoter thing. I actually found it in my textbook. If you haven't looked at 'em already, the "Genes" books by Lewin are excellent.

I know that transcriptional start sites are called promoters. My professor made reference to a "core promoter" on a day that I missed class (looking at notes from that day). I am not familiar with this terminology, can anyone help me out here?

http://www.hgmp.mrc.ac.uk/GenomeWeb/ [Broken]

Sorry, I totally misread your question there.:frown: I am not familiar with the lysosomal storage disease or glycosidases so much either, but I do know that proteases contain the potential to be quite specific based on the amino acid side chains that are contained within the peptide. Don't...

According to my molecular prof, this is referred to as trafficing. Piggybacking is is specific "type" of trafficing. We just covered the topic this week or I would have chimed in sooner.

Well according to my sources, a glycosidase is active in the degredation of sugars, while proteases break down proteins. http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=glycosidase [Broken] http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=protease [Broken]

I saw a Nova special awhile back on endostatin. The research that was behind it was amazing, as was the initial results, but I haven't heard anything more on it. A friend of mine at Purdue is doing some interesting virus work with cancer as well.

Sadly enough, I have never seen a delta symbol in front of a printed genotype. Does this mean anymore than the obvious (obvious being some given changed, unstated, to the genotype)?

I am interested in knowing if ppGpp plays a role, direct or indirect, in inhibition of DNA replication and transcription in prokaryotic cells. I can't find anything direct, but it would be my guess that in times of amino acid starvation, the cell won't actively divide, or transcribe any RNA.

I'm not sure I am following you here. Let me give you an example. the laboratory goal for this semester of my molcular biology class is to clone a fragment of the fur gene for chinese hamsters. Once the PCR product has been cleaned up, digested, and ligated into a specific plasmid, the gene...

In a microbiology or molecular biology lab for instance, when the scientiest wants to maintain the presence of foreign DNA in a system, they use antibiotic resistance genes, growing the cells in the presence of the antibiotic. So in otherwords, if you wanted the continuted expression of your...

Thank you Ian. Your input is always very much appreciated.:smile: I have just been looking for the detailed mechanism of the antibiotic on the membrane itself and can't find exactly what I am looking for.