So even though we are removing the supernatant (ethanol + drug) there might still be DCM present in that supernatant? And if we are looking at the peak at 240 nm, DCM may interfere with that peak? Also, for using a different solvent, would I want to use something with the UV cutoff lower than...
Thanks for the help! Yes the solutions are measured with a quartz 96 well plate. The standard is dissolved in ethanol with no polymer. Hmm yeah that's a good point... it may be the polymer contributing to the measurements. The polymer, however, is not soluble in ethanol. We are dissolving the...
Hey Everyone,
I'm having trouble detecting my drug dissolved in ethanol using UV-Vis spectroscopy. The peak absorbance of the drug is at 240 nm while the peak absorbance of the ethanol seems to be around 190 nm. Are these peaks too close to each other to get an accurate measurement? In the...