Main Question or Discussion Point
how MMLV RT can end labeling of DNA?It is a RNA-dependent DNA polymerase,but i read some paper use it to label dna which after Restrict enzyme digestion?? expect your reply.thx
Yes :) I think it needs to be double stranded though..Originally posted by kokoioi
thx for your reply.
It is mean MMLV RT can use DNA for template to synthesis new strand?
I am not entirely sure of your question. Are you asking whether MMLV RT needs a primer to extend the DNA?Originally posted by kokoioi
and the cleavagesite of XbaI
5'-T^C T A G A-3'
3'-A G A T C^T-5'
(the experiment is for location )
so i think MMLV RT may dont primer when it use DNA for template.
what do you think? and i can't find about MMLV RT use to label DNA in my college.
5'- C T A G A N N N N -3' 3'- T N N N N -5'