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About two-photon microscopy

  1. Nov 14, 2007 #1
    I know two-photon microscopy use the longer wavelength laser,but with more photon density.To my knowledge,the laser with more photon density means it is very strong(High Power?).But articles always say that two-photon microscopy only damage on focus point and is suitable to investigate biologic sample,while one-photon confocal microscopy will damage the sample everywhere that light can reach.

    But I really wonder,if two-photon microscopy have a stronger laser(more photon number in short time),why wouldn't it damage the sample everywhere?

    Is the laser harmful only after the sample is excited?

    Thx>.<
     
  2. jcsd
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