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Absorption spectroscopy

  1. Apr 10, 2008 #1
    Hi

    I'm trying to measure the concentration of a sample dissolved in water. I'm using the spectrometer USB4000 which directly gives me the absorbance value. Using beer-lambert law is there a way i can determine the unknown sample concentration from this measurement? I have the values 'A' and 'l' for the law. can someone throw some light on this??
     
  2. jcsd
  3. Apr 10, 2008 #2

    Andy Resnick

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    I think you need to have at elast two measurements of the absorbance with different thicknesses. There are cells for this- a rectangular cross section, so you do two readings with the cuvette rotated 90 degrees.
     
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