We had a lab tonight involving enzymes. I am concerned about two of the results we got. Test tube #1 contained: 3 ml litmus cream + 3 ml distilled water + 3 ml lipase solution Test tube #2 contained: 3 ml litmus cream + 3 ml bile solution + 3 ml lipase solution Both were placed in a 37 degree C water bath for an hour. When we pulled them out, both were a mauvish pink, and looked identical in color. Both solutions had definitely become more acidic, but seemingly to the same degree. (The litmus cream starts out bright purple but turns more red if the substance becomes more acidic and turns more blue if the substance becomes more basic.) The question I have to answer is "Which tube showed the most hydrolysis?" My thoughts are that #2 should have been more pink/acidic since the bile would emulsify the fat in the litmus cream and the lipase could more easily hydrolyze it.