Analitical chemistry

  • Thread starter RanCohen
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hi everyone,
nowadays i studing pharmaceutical engineering in 3rd year, i dont know if this is the place to ask...
my question is in hplc reversed phase chromatography, i already understand that the stationary phase is non-polar. why, when if the solvent is more polar, the retaintion time of the analyte is longer?
i asumed that is because the hidrophobic solvent taking place of the analyte and if is taking less places (if the solvent more polar) that cause the analyte interact with the stationary phase and come out from column after longer time.

Thank's ran
 

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