1. The problem statement, all variables and given/known data Prepare 500ml of a reaction buffer containing .82% MgCl2, 62.5 mM glucose, 950 ATP, and 100mM Tris -Cl, pH 7.8 Use the stock solutions you have prepared we had to prepare in previous problems: 500ml 1.75M glucose 25mL 2.5% glucose 2L of 1.8 Tris, pH 8.5 2. Relevant equations C1V1 = C2V2 Pka Tris = 8.1 glucose mol mass = 180.16 ATP mol mass = 507.8 ------------ 3. The attempt at a solution my calculation for MgCl2: .82% = .82g/100ml = 8.2g/L 15% = 15g/100ml = 150g/L C1V1=C2V2 150*x = 8.2*500 x = 28mL stck 15% MgCl2 My calculation for glucose 1.75M glucose = 315g/L 62.5mM = 11.2 g/L C1V1=C2V2 11.2*500 = 315*x x = 17.7 ml 1.75M glucose stock Here is my calculation for ATP 5mM ATP = 3g/L 950nM ATP = .00048g/L C1V1=C2V2 3*x = .00048*500 x =.08ml of stocK 5mM ATP My calculation for the Tris I am not sure about, can I use my original pH 8.5 solution the prof asked me to prepare, or do I have to start over with the Tris acid and base components and use henderson H equation? does this all look right so far?