Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Antibiotica in mammalian cells

  1. Mar 13, 2005 #1
    if my vector has an amphicilin and a neomycin selection markers. which one should i use for selection of my mammalian cells?

    thanks.
     
  2. jcsd
  3. Mar 13, 2005 #2

    iansmith

    User Avatar
    Staff Emeritus
    Science Advisor
    Gold Member

    You should understant the site of attack of the antibiotic you mention. Ampicilin attacks the cell wall synthesis whereas neomycin attacks the ribosome.

    Mammalian cell do not have cell wall. The answer is neomycin.

    Also most eukarytics cell are resitance to concentration of antibiotics used for bacteria. High concentration are required for selection.
     
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook