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Backcross vs Testcross difference: Research on mitochondrial genes
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[QUOTE="Ygggdrasil, post: 6222365, member: 124113"] In the paper, they say that the generation of the conplastic strains is described here: [URL]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612955/[/URL] Essentially, they found that different mouse strains have very similar [URL="https://www.physicsforums.com/insights/when-did-mitochondria-evolve/"]mitochondria[/URL]l genomes aside from a few mutations. They wanted to know the effects of those mutations, but to do that they can't just directly compare two different strains of mice as these mice would differ both in their [URL="https://www.physicsforums.com/insights/when-did-mitochondria-evolve/"]mitochondria[/URL]l and nuclear genomes. To isolate the effects of the [URL="https://www.physicsforums.com/insights/when-did-mitochondria-evolve/"]mitochondria[/URL]l genome, they wanted to basically put all of the different [URL="https://www.physicsforums.com/insights/when-did-mitochondria-evolve/"]mitochondria[/URL]l genomes into the C57BL/6J background. To do this, they mated a female from the mouse strain of interest with a male C57BL/6J mouse. This produces a progeny containing the [URL="https://www.physicsforums.com/insights/when-did-mitochondria-evolve/"]mitochondria[/URL]l genome of interest and about a 50% C57BL/6J nuclear genome. They then take a female progeny and mate with another male C57BL/6J. Their progeny will now have 75% C57BL/6J nuclear genome. They repeat this process for a total of 12 matings to produce a mouse retaining the [URL="https://www.physicsforums.com/insights/when-did-mitochondria-evolve/"]mitochondria[/URL]l DNA from the original mouse strain, but almost no nuclear DNA from that original strain (only about 1/2^12 or about 0.02% nuclear DNA would remain from the original strain). [/QUOTE]
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Backcross vs Testcross difference: Research on mitochondrial genes
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