1. The problem statement, all variables and given/known data Scientists isolated a clinically important protein producing gene from human cells and they want to clone it and transform into a bacterial cell, so that they can induce the protein synthesis and produce the protein commercially. But they are unable to get the protein even though all procedures are correct- they isolated the DNA then they amplified the desired gene and cloned into a bacterial vector and transformed into a bacterial cell. What may be the reason for protein is not getting produced in the bacterial cell. 2. Relevant equations NA 3. The attempt at a solution Is it that some plastmid is not able to recombine with particular gene and the same plasmid combine?