(adsbygoogle = window.adsbygoogle || []).push({}); 1. The problem statement, all variables and given/known data

I need to use the Lambert-Beer equation to plot optical density of a lactate solution.

2. Relevant equations

[tex]A=\varepsilon\ell C[/tex]

(The path length is 1 cm)

3. The attempt at a solution

This is one of my four samples (if you can show me where I'm going wrong with this then I can apply the correction to the others):

2.22 [tex]\mu[/tex]mol in 1.5ml, therefore there is 0.296 [tex]\mu[/tex]mol in 0.2ml

This info seems to match to the example graph I have.

But using the formula above, where [tex]\varepsilon[/tex]=6.22 for micromol/ml, I get:

6.22 x (0.296/0.2) micromol/ml = 9.2056

This doesn't match up with the graph I have! Any help appreciated! Please!

**Physics Forums - The Fusion of Science and Community**

# Beer-Lambert equation

Have something to add?

- Similar discussions for: Beer-Lambert equation

Loading...

**Physics Forums - The Fusion of Science and Community**