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Bio-Gel P2, cut off 2.000 daltons?

  1. Oct 2, 2004 #1
    hello everybody!

    what does it mean when a gel you use in gel filtration has a "cut off 2.000 daltons" ? i have looked for the answer, but can't find it.

    hopes for replies!

    many thanks!
     
  2. jcsd
  3. Oct 2, 2004 #2

    iansmith

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    It means that the filter will only let particle lower than (more or less) 2 dalton to pass through. Anything higher than 2 dalton will not pass.
     
  4. Oct 2, 2004 #3
    hi Ian!

    thanks for your reply, but i still don't get it. our protein (which we has to isolate) has a MW 15kDa. before we running the sample on a gel filtration, we concentrated the sample using a ULTRAFREE 15 from MILLIPORE "CUTOFF" 5kDa. as you said components at this MW or less will go through, but higher MW won't pass. then how come i concentrate my protein, when it does not pass through? :confused:


    p.s "to pass through" do you mean passing through the gel's pores?

    thanks again!
     
    Last edited: Oct 2, 2004
  5. Oct 2, 2004 #4

    iansmith

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    When you the utrafree to you add buffer to the filter or do you take the supernantant. If you add buffer/liquid to colum then the your protein did not pass the cut off.
     
  6. Oct 2, 2004 #5
    well we added our sample to this kit...

    by the way is this ULTRAFREE 15 from MILLIPORE "CUTOFF" 5kDa a gel or just a paper filter? a weird question since i did take the course! :grumpy:
     
  7. Oct 2, 2004 #6

    iansmith

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  8. Oct 3, 2004 #7
    thanks, Ian!
     
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