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BIO: questions about GFP and CaM

  1. Feb 17, 2009 #1
    1. The problem statement, all variables and given/known data

    The molecule in question is GFP with CaM
    1) Suggest several point mutations that will disable the protein's function without dramatically altering the protein's shape. Explain why.
    2) Suggest a point mutation that will completely alter the protein's shape. Explain why.

    2. Relevant equations

    3. The attempt at a solution

    1) I'm guesssing the main function of the protein right now is to detect Ca2+. I read somewhere else that it can detect certain proteins afterwards too, but i'm not sure which is more accurate...so I think mutating CaM at the Ca2+ binding site by changing the negative amino acids to positive ones will work? Again, i'm not exactly sure what the point mutations are on the EF hand...and I'm not sure whether that will change the shape by a lot...
    2) a single point deletion will cause a frameshift mutation (i'm not sure if this counts as a point mutation though?) which will cause a different sequence of amino acids to be linked, hence the shape will be very different.
  2. jcsd
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