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Biochemistry (Enzyme Kinetics)

  1. Oct 9, 2006 #1
    The following kinetic data were obtained for an enzyme in the absence of inhibitor (trial A), and in the presence of two different inhibitors (B and C) at concentrations of 5 mM


    a) Determine Vmax and Km for the enzyme (Assume [Et] is the same for each trial)

    the first thing I thought to do was use the relationship that Km = when Vo= 1/2Vmax, however, after posing this question to my professor that is incorrect. I really don't know where to start so any help to get me started on the right track would be immensely appreciated.
    Last edited: Oct 9, 2006
  2. jcsd
  3. Oct 10, 2006 #2


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    Hi, you need to read through the relevant chapter in your text a bit more carefully, it should describe a specific method of plotting to obtain the Vmax and Km hint: This method is probably one of the most important concepts explained within the chapter of which it is mentioned, the Vmax can be obtained from the y-intercept value of the unique plot and the x-intercept should reveal the Km.
  4. Oct 10, 2006 #3
    ah yes Lineweaver-Burk plot, thanks
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